Bone Wind Tablets HSYA A Determination.docVIP

 PAGE \* MERGEFORMAT 7 Bone Wind Tablets HSYA A Determination [Abstract] Objective: To establish bone wind Tablets HSYA A method for the determination. Methods: The high-performance liquid chromatography (HPLC), Shim  packC18 column (250mm * 4.6mm, 5μ m), using methanol -0.4% phosphoric acid (32:68) as mobile phase, detection wavelength 403nm, flow rate 1.0ml / min. Results: HSYA A within the 0.1032-0.516μ g showed good linear relationship, r = 0.9999, average recovery was 98.517%, RSD of 1.730%. Conclusion: The method is simple, accurate, and can be used as bone Wind Tablets HSYA A quantitative analysis method. [Keywords:] bone Wind Tablet; HSYA A; high performance liquid chromatography Feng Ning piece of bone from the re-building, Clematis, astragalus, red sage root, safflower, etc. composed of 11 Chinese herbal medicines compound preparation, with Jieduhuayu, reviving the pain and other effects. In this paper, high performance liquid chromatography (HPLC) on HSYA A conducted a Determination. The method is simple, accurate, specific and strong. An instrument and reagent 1.1 Instrument performance liquid chromatography (Shimadzu, Japan), LC  10ATVP type, SPD  10AVP UV detector, 7725i manual injector, UV spectrophotometer, UV  3000, Japan Shimadzu. 1.2 Drugs and reagents HSYA A reference substance, batch number: 111637  200502, for the determination of use, drugs and biological products from China provided by, methanol, HPLC pure, other reagents were of analytical grade. 2 Methods and Results 2.1 high-performance liquid chromatography conditions and system is suitable for testing Shim  pack C18 (150mm * 4.6mm, 5μ m); with methanol -0.04% phosphoric acid (32:68) as mobile phase; detection wavelength of 403nm. Number of theoretical plates by HSYA A calculation should not be less than the 3000 peak. 2.2 The product solution for the test preparation and study methodology 2.2.1 Preparation of reference substance solution precision Weigh HSYA A