Brain memory source capsule Determination of flavonoids.docVIP

 PAGE \* MERGEFORMAT 12 Brain memory source capsule Determination of flavonoids Of: Deng Ruiyun, Yonggang, Zhou Lin to change, district Ji-ming, Lin Li, Cheng Bo, Li [Abstract] [Objective] To establish the source capsule in the brain memory of total flavonoids and method for determination of icariin. [Method] lt;”Health food inspection and evaluation of technical specificationsgt;” (2003 edition provides the means to rutin as reference, the use of ultraviolet - visible spectrophotometry of total flavonoids, high performance liquid chromatography (HPLC method for the determination of icariin in capsules contents of the column: luna Cl8 (2 100R (150mm 4.6mm, 5m, mobile phase : acetonitrile - water (volume ratio of 30:70, flow rate: 1.0mL/min, detection wavelength: 270nm. [results] rutin at 0 ~ 24.75g / mL range was a good linear relationship, r = 0.9990, average recovery was 102.29%, sR is 1.34% (N = 5, icariin content in 6.9 10-2 ~ 4.83 10-1g peak area within the range of values was linear, r = 0.9999 The average recovery was 95.39%, sR was 2.5% (N = 5. [Conclusion] The developed method can accurately determine the source capsule in the brain recalled the content of flavonoids. [Keywords:] brain memory source capsule / chemistry, total flavonoids / analysis, icariin / analysis, chromatography, high pressure liquid, spectrophotometry, ultraviolet Abstract: ObjectiveTo establish a method for the determination of total flavonoids and icariine in Naoyiyuan Capsules. MethodsWith rutin as reference, total flavonoids was determined by ultraviolet spectrophotometer with the method which was recorded in Health Food Test and Evaluation Technological Specification (2003 edition. The content of icariine in Naoyiyuan Capsules was detected by HPLC. The chromatographic separation was performed on Luna C18 (2 100R column (150mm 4.6mm, 5m with mobile phase consisted of acetonitrile water (30:70 at a flow rate of 1.0 mL / min. The detection wavelength was set at 2