Breast cancer BA46 gene transfer of dendritic cells induced specific cell-mediated immunity.docVIP

 PAGE \* MERGEFORMAT 4 Breast cancer BA46 gene transfer of dendritic cells induced specific cell-mediated immunity [Abstract] Objective To explore the adeno-associated virus as a carrier, breast antigen BA46 gene transfected dendritic cells (DCs) induced specific cell-mediated immunity is feasible. Methods to remove the healthy human peripheral blood, using density gradient centrifugation method of separation of peripheral blood mononuclear cells (DCs precursor cells) to AIM  V train based on 6-well plates cultured adherent 5h, gently wash away the suspension cells, pick-and - wall cel