Chinese herbal compound on advanced glycation end products on cultured mesangial cells under proliferation and hyperplasia of mesangial matrix.docVIP

 PAGE \* MERGEFORMAT 11 Chinese herbal compound on advanced glycation end products on cultured mesangial cells under proliferation and hyperplasia of mesangial matrix Of: Wang Qiong, Wang Ning, Yu Li, Chen Lijun, Zhu Quan [Abstract] Objective To observe the effect of compound on advanced glycation end products (AGEs) develop under the mesangial cell proliferation and extracellular matrix secretion. Methods MTT assay of mesangial cell proliferation, alkaline hydrolysis Detection of cell content of hydroxyproline in the supernatant. Results 1 × 10-3,1 × 10-2,1 × 10-1 mg / mL extract of Chinese medicine compound inhibited rat cultured in glomerular AGEs mesangial cell proliferation, to reduce the role of AGEs in the supernatant under the hydroxyproline content, inhibit the proliferation of mesangial matrix. Conclusion Chinese herbal compound can inhibit the AGEs induced mesangial cell proliferation and mesangial matrix proliferation, suggesting that the party may prevent the AGEs receptor signaling pathway by inhibiting the development of diabetic nephropathy. [Keywords:] advanced glycation end products, mesangial cell proliferation; mesangial matrix proliferation, diabetic nephropathy, traditional Chinese medicine Abstract: Objective To observe the effect of the prescription on the progress of mesangial cell proliferation and matrix secreted induced by AGEs. Method The proliferation of glomerular mesangial cell cultured in media was measured by MTT. Hydroxyproline level was measured by alkaline hydrolysis. Result The proliferation of mesangial cells were obviously increased by AGEs (0.25 mg / mL). The proliferation mesangian cells and the hydroxyproline levels were suppressed by the prescription of the concentration 1 × 10-3, 1 × 10-2, 1 × 10-1 mg / mL. Conclusion The prescription could suppress the proliferation of mesangial cells and extracellular matrix induced by AGEs. It suggested that the prescription play a significant role in the prevention of