Chitosan reconstruction of peripheral nerve function in the role of.docVIP

 PAGE \* MERGEFORMAT 7 Chitosan reconstruction of peripheral nerve function in the role of On: Sun Zhenbo Xiangying Xu Xiaoyi Cui Wen Wei Li Wei Wang Guanjun long [Keywords:] nerve injury; chitosan; biodegradable; polymer materials Nerve damage is common disease in elderly patients, often resulting in sensory dysfunction and in patients with limited mobility, or even lose the ability to work. Therefore, improving the speed of nerve repair and reconstruction to accelerate tissue regeneration and promote functional recovery, is an important research subject geriatrics (1, 2). In this study, anti-inflammatory, promoting the growth of the natural polymer chitosan with absorbable caprolactone / lactide (PLCL) composite films as a hollow pipe to guide nerve regeneration, nerve bridging the two ends, neurons can be recycled through the lumen, to achieve the purpose of repairing damaged tissue. 1 Materials and methods 1.1 Preparation PLCL (molecular weight 100,000) in accordance with 8 wt% dissolved in chloroform. The solution in an ice bath, under high shear emulsification, drop by drop chitosan (molecular weight of 4500, degree of deacetylation 99%) acetic acid solution to the chitosan in PLCL uniform distribution, the chitosan mass ratio with PLCL 1:1,1:5 and 1:10, respectively. salivation prepared using thin film, vacuum drying 48 h, to remove residual solvent to obtain a thickness of 10 ~ 20 m film. cut to appropriate size sheet, ethylene oxide sterilization, dry place for use. 1.2 The analytical method of chitosan-PLCL electron microscopy surface morphology. The water contact angle to detect the proportion of chitosan-PLCL thin films hard surface. At room temperature, stretching rate of 5 mm / min under the conditions Determination of tensile properties of materials. application of MTT analysis of chitosan-PLCL and PLCL L929 mouse fibroblasts on the toxicity evaluation of material biocompatibility. 1.3 Animal experiments 27 SD rats (weight 20