Chronic Obstructive Pulmonary Disease and Lung-Yin deficiency syndrome patients with T lymphocytes comparative analysis of gene expression.docVIP

 PAGE \* MERGEFORMAT 15 Chronic Obstructive Pulmonary Disease and Lung-Yin deficiency syndrome patients with T lymphocytes comparative analysis of gene expression Of: with Fei, Peng Bo, Zhang Nian Zhi, Zheng Seoul, Ji Hongyan [Abstract] Objective To study gene chips with chronic obstructive pulmonary disease (COPD) Patients with Deficiency of Qi Deficiency and Yin T lymphocyte gene expression differences. Methods We collected Qi deficiency, Yin deficiency peripheral blood of patients and healthy people blood samples as the experimental and control blood samples. Peripheral blood lymphocytes were separated by Ficoll, type separation by flow cytometry with sorting and purification of T lymphocytes, and uses one-step isolation of total RNA, synthetic double-stranded cDNA after reverse transcription, in vitro transcription synthesis of biotin-labeled cRNA, After fragmentation, the use of human whole gene chip for chip hybridization and scanning of differentially expressed genes were screened out. Results Qi Deficiency patient / human peripheral blood T lymphocyte-associated differentially expressed genes 45, which increased 41 down 4 Article, Lung-Yin Deficiency Syndrome-related peripheral blood T lymphocytes differentially expressed genes 32, which increased 19, down 13, Qi Deficiency group / healthy group and Yin deficiency group / healthy groups were highly expressed in differences in gene 5. Conclusion gene chip technology to effectively study gene expression profile, screening out the COPD lung deficiency, Yin deficiency between patients with T lymphocyte-associated genes. [Keywords:] chronic obstructive pulmonary disease, lung deficiency, Yin deficiency, T lymphocyte, gene expression, gene chip Abstract: Objective To study the associated gene expression in thymus-dependent lymopholyte of the COPD patients with pulmonary qi deficiency syndrome (PQDS) and pulmonary yin deficiency syndrome (PYDS) by gene chips. Methods Collect peripheral blood of pa