Circular dichroism spectroscopy study of environmental factors on cells of the red protein secondary structure.docVIP

 PAGE \* MERGEFORMAT 14 Circular dichroism spectroscopy study of environmental factors on cells of the red protein secondary structure Of: Tiansu Yan Gao Lei Mu Lian-of-phase construction of Tan Shi Shuai Liu Xiaoyan [Abstract] far-UV circular dichroism spectroscopy system to study red cell protein (cytoglobin, Cygb) concentration, temperature, pH value and solvent properties on protein secondary structure of red cells. The results show that: when Cygb concentrations lt;1 μmol / L, it exists mainly in the form of @ helix, the @ helical contentgt; 60%; when Cygb concentration from 0.3 μmol / L increased to 2.0 μmol / L, its @ helix content decreased rapidly; when Cygb concentrationgt; 2.0 μmol / L, its @ helix content changed little with increasing concentration (30%). as the temperature rises, Cygb the @ helix content decreased, but even if the temperature reaches 368 K It remains @ 20% helical structure, indicating that the protein has a high thermal stability. in weak acid and weak alkaline solution, Cygb secondary structure will have varying degrees of damage. in methanol and ethanol , Cygb’s @ helix content increased significantly, indicating that alcohol can induce the formation of the @ helix. [Keywords:] red cell protein, circular dichroism, secondary structure, @ helix 1 Introduction Red cell protein (cytoglobin, Cygb) was recently found in vertebrate tissue globins 4 [1], it has the typical globin “three over three” types of @ helix sandwich fold structure, but in the E helix His81 is coordinated with the heme, formation and NGB (neuroglobin, Ngb) similar to the six-coordinated heme [2,3]. Cygb various tissues in the body is almost always expressed, but its exact biological function It is not entirely clear. research shows that, Cygb may have oxygen, oxygen storage, oxygen sensors and the peroxidase function, in addition it may also be related to the formation of collagen [4]. At present, the Cygb spatial structure, dynamic characteri