Cirrhotic rat liver pathology and hemodynamics.docVIP

 PAGE \* MERGEFORMAT 13 Cirrhotic rat liver pathology and hemodynamics Of: Qiao Wei, Lu Jianguo, Wang Qing, Li Chao [Abstract] Objective To establish a rat cirrhosis model, to understand the process of liver cirrhosis portal hemodynamics changes. Methods 100 healthy male SD rats were randomly divided into 5 groups: 4 experimental groups (80 rats in each group 20) by intraperitoneal and subcutaneous injection of carbon tetrachloride (CCl4), also were induced to drink alcohol solution; control group (20) to give ordinary drinking water and feed. were 2,4,7,10 weeks after the liver histopathological changes and changes in portal hemodynamics. Results of liver cirrhosis of the liver cells through the process of degeneration, necrosis, fibrosis and the formation of false lobules; Hemodynamics indexes corresponding changes in mean arterial pressure (MAP) showed gradual decreased portal vein pressure (PVP), inferior vena cava pressure (IVCP) and portal vein resistance (PVR) showed increased gradually, portal venous flow (PVF) at first and then decreased, splanchnic vascular resistance (SVR) at first and decreased and then increased. Conclusion The method can create a stable model of cirrhotic portal hypertension, and is suitable for mass production; in the process of liver cirrhosis portal hypertension portal hemodynamics in liver tissue pathology have occurred in changes, can be used for aspects of portal hypertension research. [Keywords:] liver cirrhosis; portal hypertension; histopathology; hemodynamics; rat ABSTRACT: Objective To investigate the hemodynamic changes during the progression of portal hypertension (PHT) by establishing a model of liver cirrhosis in rats. Methods Totally 100 healthy male SD rats were assigned to 5 groups randomly (1 control group and 4 experimental groups with 20 rats in each group). Animal model of cirrhosis was established by subcutaneous injection of carbon tetrachloride (CCl4) and drinking alcohol. Rats in control group