Cloning of typecollagen and its application in normal and age-related cataract lens epithelial cells.docVIP

 PAGE \* MERGEFORMAT 3 Cloning of typecollagen and its application in normal and age-related cataract lens epithelial cells Author: Liu Yan Zhou Liu Kuan-ping YAO Li-Bo SU Yan-Nian Hui Jing [Keywords:] collagen Cloning of collagen type Ⅰ gene and its expression in lens epithelium in normal and agerelated cataractous people [Abstract] AIM: To clone the gene of collagen type Ⅰ , to study its expression in lens epithelium of normal and agerelated cataractous people, and to compare the difference of their expression. METHODS: The collagen type Ⅰ gene was cloned by the improved subtractive hybridization, and the fragment of collagen type Ⅰ cDNA was labeled by digoxin and was hybrided by in situ hybridization on the section of capsule of normal and cataractous lens. RESULTS: Collagen type I gene expressed both in the normal and the agerelated cataractous lens epithelium. The positive cell rate was respectively (73 ± 149)% and (75 ± 163)%, the positive signals index was respectively (060 ± 015) and (073 ± 016) and the expression of the two groups had no significant statistics difference. CONCLUSION: Collagen type Ⅰ gene is expressed in the normal and the agerelated cataractous lens epithelium. There is no significant differences in the expression of collagen type Ⅰ gene in the two groups, indicating that collagen type Ⅰ gene may play an important role in the etiology of cataract and the formation of posterior capsule opacification after cataract surgery. [Keywords] collagen; RNA, messenger; cataract; lens; epithelial cells [Abstract] Objective: To clone genes and study of type Ⅰ collagen in normal age-related cataract lens and lens epithelial cells, and compare the two differences in the expression. Methods: The modified subtractive hybridization cloning of type Ⅰ collagen genes, to Digoxigenin-labeled cDNA fragment of type Ⅰ collagen production of probes, in situ hybridization to study the slices in the anterior lens capsule on the expression and t