Combined captopril and losartan on myocardial infarct zone Comparison of the effects of early reconstruction.docVIP

 PAGE \* MERGEFORMAT 2 Combined captopril and losartan on myocardial infarct zone Comparison of the effects of early reconstruction Authors: Zhang Xiaoxia, Wu Xue-si, Han Zhihong, rice Shu-hua [Abstract] Objective: To investigate the acute myocardial infarction (AMI) after combined application of early captopril and losartan on infarct collagen deposition and infarct expansion in extent and its mechanism. Methods: 56 male Wistar rats thoracotomy ligation anterior descending coronary artery to establish AMI model, within 24 h after operation were randomly divided into model group, captopril group, captopril + losartan group (combination group). captopril 2 g / L dissolved animals, drinking water, free drinking water; losartan 20 mg / (kg * d) gavage. 14 d after intravenous injection of 100 g / L potassium chloride to the heart diastolic cardioplegia, neutral formaldehyde, and constant pressure perfusion fixation left ventricle, direct measurement of ventricular volume to do post-production cross-section of left ventricular biopsy. OK HE staining and image analysis software with the medical determination of infarct size, infarct expansion index; immunohistochemical staining was determined infarct zone of collagen type I surface density, the proportion of muscle fiber cells, ; 6 rats in each group an alternative to RT  PCR determination of infarct type Ⅰ , Ⅲ collagen expression. Results: The combination of captopril or losartan were significantly reduced infarct expansion index, but the combination group infarct expansion the index to improve the inferior to captopril group [(1.25 ± 0.13 vs 1.12 ± 0.14), P