Combined with adriamycin and curcumin enhance the human leukemia cell line HL60 the sensitivity to doxorubicin.docVIP

 PAGE \* MERGEFORMAT 8 Combined with adriamycin and curcumin enhance the human leukemia cell line HL60 the sensitivity to doxorubicin Author: Lei Ke-Hong Wang Ren-ming, a feather [Abstract] Objective To study whether curcumin to enhance human leukemia cell line HL  60 the sensitivity to doxorubicin and its mechanism. Methods MTT determination of drugs in vitro, using flow cytometry analysis of apoptosis. Application of RT  PCR and Western blot detection of Survivin and XIAP gene expression. Results Curcumin (2.00 ~ 16.00 μ mol * L-1) and adriamycin (0.08 ~ 0.64 μ mol * L-1) at the same time administration of the HL-60 cells can produce enhanced synergistic killing effect, at the same time, apoptosis increased, decreased the expression of Survivin and XIAP. Conclusion The combined application of adriamycin, and curcumin can enhance the human leukemia cell line HL  60 the sensitivity to doxorubicin, down survivin (Survivin) and X-associated inhibitor of apoptosis protein (XIAP) expression in the promotion of apoptosis is its the main mechanism of action. [Keywords:] curcumin; doxorubicin; synergies; survival protein; X-related apoptosis-inhibitory protein Abstract: ObjectiveTo investigate the effect of curcumin on the sensitivity of human leukemia cell line HL-60 to adriamycin. MethodsCytotoxicity was determined by the colorimetric MTT viability / proliferation assay. Apoptosis was assessed by flow cytometry. The mRNA levels of survivin and XIAP were measured by RT-PCR. The protein levels of survivin and XIAP were measured by Western blot. ResultsSimultaneous administration of curcumin (2.00 ~ 16.00 μ mol * L-1) and adriamycin (0.08 ~ 0.64 μ mol * L-1) resulted in synergistic cytotoxicity and apoptosis for HL-60 cells. Meanwhile, cell apoptosis was increased and the mRNA and protein levels of Survivin and XIAP were significantly down-regulated. ConclusionCurcumin can sensitize human leukemia cell line HL-60 to adriamycin-mediated apoptosis. Down-reg