Compounds inhibit chitinase-producing strains of.docVIP

 PAGE \* MERGEFORMAT 4 Compounds inhibit chitinase-producing strains of Author: Xie Jie ZHOU Ze-Yang ZHANG Xin-jun Blue Greek clamp HU Jun-hua [Keywords:] Chitinase;, inhibiting compounds;, producing bacteria Abstract: From the nation more than 200 were collected from soil samples were isolated more than 1350 strains of bacteria, using Plate Culture Method and iron potassium cyanide methods to silkworm midgut chitinase as a target from these strains were screened out of Silkworm chitin inhibitor compound-producing strain 1237 #, after preliminary identification of the strain of Pseudomonas. This paper describes chitinase inhibitor compound screening system for the establishment of active substances to explore the nature and purpose of the preliminary identification of strains. Keywords:: chitinase; inhibiting compounds; producing bacteria Preliminary screening of chitinase inhibitor producing strains ABSTRACT In the course of screening for new chitinase inhibitor, using the silkworm chitinase as a target, and transparent inhibition zone plate assay and potassium ferricyanide method, a strain belonging to the Pseudomonas, named # 1237 was isolated among 1350 strains from 200 soil samples. The screening procedure and the preliminary study of the culture were also reported. KEY WORDS Chitinase; Inhibitor; Producingstrain Chitin is crustacean, insect exoskeletons, and an important component of fungal cell wall, which is N-acetyl glucosamine with β 1, 4 keys connected to straight-chain polymer. Chitin in nature, second only to the presence of the amount of cellulose in second place. Chitinase is a kind of able to degrade chitin N-acetyl amino glucose or oligo-N-acetyl amino glucose hydrolase. Fungi need to chitinase degradation of picture-compartment between cells in order to complete their reproductive process; insects during development also need to chitinase partially degraded exoskeletons in order to complete its process of metamorphosis, so the reproduc