Cornel Polysaccharide concentration on the senescence of HDF cells.doc

 PAGE \* MERGEFORMAT 11 Cornel Polysaccharide concentration on the senescence of HDF cells Of: parsley Zhanghui Dan ZHANG Kun Huang Jing Piao Jin-Hua Qiu [Abstract] Objective To study the concentration of dogwood polysaccharides on human fetal lung in vitro diploid fibroblasts (HDF) explore the effect of polysaccharide rabbit serum prepared with the best dogwood blood collection time. Methods targeting rabbits, prepared dogwood polysaccharide serum, DMEM culture medium cell replicative senescence HDF; by phenol sulfuric acid method polysaccharide content, MTT cell viability assay. the HDF cells were divided into 4 groups, treated with different concentrations of polysaccharide rabbit serum medium containing Cornus HDF on Aging cell activity. The results of serum concentration of Cornel Polysaccharide 1.5,2,4 h was significantly higher than in the administration of medication 0.5,11 h (P lt;0.001), higher than the administration 2,2.5,3,3.5,4 h 0.5,11 h (P lt;0.01), while in the administration of 48,72 h was significantly lower (P lt;0.01). medium with rabbit serum containing dogwood polysaccharides (SCP) 0.5%, 1% and 1.5% could increase old HDF cell viability (P lt;0.01), cell aging phenotypes is not obvious, in which 1% SCP group was better than 0.5%, 1.5% group. and 2% SCP group and 3% SCP group showed significant inhibition of cell growth. Conclusion Preparation of polysaccharide rabbit serum containing dogwood best time in the blood after administration of 1.5 ~ 2 h or 4 h, dogwood on Anti aging effects of HDF cells is the best dogwood polysaccharide containing 1% rabbit serum group. [Keywords:] Cornel Polysaccharide; human embryonic lung diploid fibroblast; aging; serum Dogwood with liver or kidney, Yijing gas, lowering blood sugar, antibacterial, diuretic, blood pressure and immune function (1). Pharmacological studies show that the dogwood has antioxidant, anti-experimental liver injury and stimulate the immune response, anti-rheumatoid infla