COX2 and VEGF with liver cancer multicellular spheroids the relationship between the cluster resistance.docVIP

 PAGE \* MERGEFORMAT 2 COX2 and VEGF with liver cancer multicellular spheroids the relationship between the cluster resistance Author: YAO Yu, Tian Tao, Cui Jie, Na Zhao, Guo Yahuan, Nancke Jun [Abstract] Objective: To investigate COX  2 and VEGF in hepatocellular carcinoma multicellular spheroids (MCS) in the expression of liver cancer cluster resistance. Methods: The liquid overlay technique of HepG2 liver cancer multicellular spheroids as a model, using transmission electron microscopy and scanning electron microscopy comparison of the morphology of single cells and MCS differences; using MTT method and flow cytometry compare ADM and 5  FU pairs of single cells and MCS of the growth inhibition rate and apoptosis rate; using immunohistochemical staining and Comparison of image analysis techniques COX  2 and VEGF in the monolayer cells and multicellular spheroids differences in the expression. Results: Compared with monolayer cells, multicellular spheroid cell adhesion extensive and close connections and bridges between grains can be seen to connect. different concentrations of of ADM and 5  FU role of 48 h, MCS cell growth inhibition rate and apoptosis rate were lower than the monolayer cells, the difference was statistically significant (P