D galactose combined half-fat diet-induced cognitive dysfunction in rat model and evaluation of.docVIP

 PAGE \* MERGEFORMAT 21 D galactose combined half-fat diet-induced cognitive dysfunction in rat model and evaluation of [Abstract] Objective To establish the complex factors that caused cognitive dysfunction in rats, and from behavioral, pathological morphology, apoptosis, oxidative damage and inflammatory factors such as the angle evaluation. Methods and normal female SD rats were used subcutaneous injection of D  galactose and semi-fat diet feeding, preparation 6 w and 8 w not accompanied by processes of cognitive dysfunction animal model. Using Morris water maze test and object recognition test to detect the capacity of learning and memory in rats; using HE staining and electron microscopic observation of pathological changes in hippocampus; determination of brain malondialdehyde (MDA), oxidized low-density lipoprotein (ox  LDL), Tumor necrosis factor  α (TNF  α ) and interleukin  6 (IL  6) of the content; less cognitive dysfunction at the same time range and between rats and normal rats difference. Results 6 w at the end, the rat model of cognitive dysfunction in object recognition experiment, to distinguish index decreased significantly compared with the control group (P amp;lt;0.05), but the water maze test results with the control group showed no significant difference; and 8 w, when the end of the model animals to distinguish index decreased (P amp;lt;0.01), while a marked decrease of water maze performance, escape latency was significantly prolonged (P amp;lt;0.01), significantly reduced the number of clothing units (P amp;lt;0.05); 8 w and 6 w end of the end of compared with water maze test was significantly longer escape latency (P amp;lt;0.01); After modeling, can lead to degenerative changes in hippocampal nerve cells and early apoptosis, and there is progressive increase of the pathological changes; 6,8 w model rat brain tissue TNF  α , IL  6 levels were significantly higher (P amp;lt;0.05, P amp;lt;0.01), while 8 w rat IL  6 l