Dan Koukang on betel nut extracts on human oral mucosal stimulation of fibroblast proliferation and expression of proliferating cell nuclear antigen.docVIP

 PAGE \* MERGEFORMAT 12 Dan Koukang on betel nut extracts on human oral mucosal stimulation of fibroblast proliferation and expression of proliferating cell nuclear antigen Of: Liyuan Cong Tan Jin Chen Peng Chuxiang [Abstract]: Objective View of Chadan Koukang extract of betel nut (Areca Nut Extract, ANE) induced human oral mucosa fibroblasts (Fibroblasts, FB) proliferation and PCNA (Proliferating cell nuclear antigen, PCNA) expression . Methods in cultured human oral mucosa FB, 100 μg / mL ANE as the induction agent, 10% rat serum Dan Koukang materials for the intervention, with dimethyl thiazolyl diphenyl tetrazolium bromide (MTT) method cell proliferation, detected by immunocytochemistry the expression of PCNA. MTT results showed that Dan Koukang ANE group OD value lower than the stimulation group (P lt;0.01 or P lt;0.05), Dan Koukang group of immunoreactive cells and the relative gray value were lower than ANE stimulation group (P lt;0.01 or P lt;0.05). Conclusions Dan Koukang ANE stimulation can inhibit the proliferation of human oral mucosa FB, reduced the expression of PCNA. [Keywords:] Dan Koukang betel nut extract fibroblast proliferation cell nuclear antigen Abstract: Objective To study the effect of Danxuan Koukang (DXKK) drug serum on proliferation and proliferating cell nuclear antigen (PCNA) production of human oral mucosal fibroblasts (FB) induced by areca nut extract (ANE). Methods Human oral mucosal FB were cultured in vitro, 100 μg / mL ANE was used as inducement and 10% DXKK drug serum as intervenor. The cell proliferation rates were measured by MTT assay, PCNA productions were examined by immunocytechemistry. Results MTT assay showed OD value in DXKK groups were significantly decreased compared with the induced group (P lt;0.01 or P lt;0.05). Immunocytechemistry showed counts and relative grey value of PCNA-positive cells in DXKK groups were significantly decreased compared with the induced group (P lt;0.01 or P lt;0.05). Conclus