Dermal multipotent stem cells combined with polylactic acid scaffold repair full-thickness articular cartilage defects in rabbits.docVIP

 PAGE \* MERGEFORMAT 19 Dermal multipotent stem cells combined with polylactic acid scaffold repair full-thickness articular cartilage defects in rabbits Of: Counterattack one Lumao Sen, Yuan Lingwei, Xing Shuai, Jing Wang, BRANCH UNDER, Han Hua, Shen Haili [Abstract] [Objective] To investigate dermal multipotent stem cells in cartilage defects in the feasibility for the expansion of cartilage defects in the selection of seed cells to provide experimental basis. [Method] newborn pigmented rabbits as the research object, direct separation of mechanical method dermal tissue obtained using cells obtained by enzymatic digestion, adherent stem cells to obtain high adhesion characteristics of the growth of clonal cell populations, and were subcultured. so that cell concentration of 1 × 106/ml and polylactic acid scaffold week after the plants were cultured into the rabbit full-thickness defects of articular cartilage, with 3 to 5-month-old Chinchilla rabbits 30, 60 joints were randomly divided into the dermal multipotent stem cell / polylactic acid scaffolds, polylactic acid scaffold, the three control groups n = 20 joints of air embolism at 12 weeks rabbits were sacrificed on the repair tissue were drawn, the line HE, toluidine blue stain, according to the repair of cartilage defects in the gross and histologic scores were statistically analyzed and compared whether the groups were statistically significant differences in scores. [Results] gross and histological observation showed dermal stem cell / scaffold smooth surface repair tissue in experimental group, were hyaline, with the surrounding cartilage and subchondral bone well-integrated, and polylactic acid scaffold group, some hyaline cartilage, mainly in the fibrous cartilage repair, the blank surface defects in the control group, fibrous cartilage repair. gross and histological scores after the statistical analysis shows that the best A group fixed effects, than B, C group (P lt;0.05, B group than C grou