Determination Guizhifuling Paeonol Capsules.docVIP

 PAGE \* MERGEFORMAT 5 Determination Guizhifuling Paeonol Capsules [Keywords:] Fuling capsule; paeonol; RP-HPLC Fuling capsule by moutan, cassia twig, Poria and other refined from pure Chinese medicine. This product can reduce blood viscosity, inhibiting platelet aggregation, on a certain relaxation of rat uterine smooth muscle function, and small mice have analgesic and anti-inflammatory effect. Guizhifuling capsule blood stasis, ease Xiaozheng block. In this study, due to the lower abdomen for the treatment of women with blood stasis syndrome places a block or leave out more than menstrual volume, dark purple color , more blood clots, abdominal pain or abdominal pain, refused to press, dark tongue with ecchymosis, astringent or thin veins. paeonol one of its active ingredients, using RP-HPLC with Paeonol Guizhifuling capsule content. 1 apparatus and materials 1.1 Instrument Company PC 3000 SSI HPLC, UV-300 spectrophotometer thermoelectric. 1.2 paeonol standard reagent (Pharmaceutical and Biological Products, batch number: 1107082004); Fuling Capsule (Jiangsu Kang Yuan Pharmaceutical Co. Ltd., batch number: 061107,060916,061228); methanol (excellent grade alcohol). 2 Methods and Results 2.1 Chromatographic conditions Shimadzu Kromasil C18 column (4 m, 4.6 mm 250 mm), mobile phase of methanol water (60:40), detection wavelength was 274 nm, column temperature 30 [1], flow rate 0.18 ml min- 1, AUFS 0.05, the injection volume was 10 l, the retention time of paeonol 7.84 min, data processing for the external standard peak area method. chromatogram shown in Figure 1. a standard chromatogram paeonol; b Guizhifuling capsule chromatogram; c blank reference substance; a peak paeonol Figure 1 RP HPLC chromatogram 2.2 Preparation of standard curve to take a certain amount of precision that paeonol reference substance, were made with distilled water 25.02,50.04,75.06,100.08,150.02,250.2 g ml-1 standard solution, each sample 10 l, the peak