Determination of clenbuterol in pig urine residue enantiomers.docVIP

 PAGE \* MERGEFORMAT 15 Determination of clenbuterol in pig urine residue enantiomers Of: Wuyin Liang Ji Hao Yang Ting Shan Weiguo Huangfu [Abstract] for the determination of clenbuterol in pig urine residue enantiomers by high performance liquid chromatography method. In alkaline conditions, extracted with ethyl acetate 10 mL pig urine sample extract was diluted Anti-HCl extraction, extract directly over the SCX solid phase extraction column, and then 5% ammoniated methanol elution, the eluent is dried by nitrogen gas to volume with 200 μL methanol. by Astec CHIROBIOTICTM V chiral column, V (methanol: V (acetic acid: V (= 99.94:0.02:0.04 triethylamine as mobile phase HPLC analysis, detection wavelength 301 nm, external standard. clenbuterol single enantiomer peak area and concentration In 70 ~ 5000 μg / L was linear within the range, the linear correlation coefficient greater than 0.9996, pig urine samples the detection limit was 0.30 μg / L. pig urine clenbuterol enantiomers in 1.0 20.0 μg / L within the scope of recovery was 76.3% ~ 91.5%, relative standard deviation RSD were less than 7% (n = 5). [Keywords:] clenbuterol, enantiomers, HPLC, pig urine Abstract A method was developed for the determination of residual clenbuterol enantiomers in swine urine by high performance liquid chromatography. A 10 mL sample was extracted with ethyl acetate under basic condition. Then, clenbuterol enantiomers were stripped from the extract using diluted hydrochloric acid to remove the fat. The hydrochloric acid extract was purified by SCX solid phase extraction (SPE) cartridge. The eluent was dried by nitrogen and then dissolved in 200 μL methanol. The samples were analyzed on a chiral column (Astec CHIOBIOTICTM V) with a mixture of methanol acetic acid triethylamine as the mobile phase and quantified with the external standard calibration curve method. The detection wavelength was set at 301 nm. Good linearities were obtained for the two enantiomers at the con