Determination of omeprazole in the flow injection chemiluminescence method.docVIP

 PAGE \* MERGEFORMAT 19 Determination of omeprazole in the flow injection chemiluminescence method [Abstract] Objective To determine the flow of omeprazole injection chemiluminescence method. Methods omeprazole When injected into the combined reaction of potassium permanganate and luminol stream, it can detect the CL signal intensity with flow injection technique on the experimental conditions were optimized. results in the optimum conditions, ie, mixing tube length: 150cm, the reaction medium alkalinity: 0.15mol / L NaOH, luminol concentration: 7.0 10-5mol / L, potassium permanganate concentration: 2.0 10-5mol / L and the reagent flow rate: 2.8mL/min, omeprazole concentration 2.0 10-8 ~ 1.0 10-5g/mL in the range of the relative chemiluminescence strength of a linear relationship; on the concentration of 1.0 10-7g/mL and 1.0 10-6g/mL omeprazole solution was 11 determinations of the relative standard deviations were 1.0% and 2.6%; the method of inspection the limit is 3 10-9g/mL. Conclusion The method is simple, fast, accurate and suitable for pharmaceutical preparations in the determination of omeprazole. [Keywords:] omeprazole; chemiluminescence; Flow injection; drugs ABSTRACT: Objective To develop a new flow injection chemiluminescence (FI CL) method for the determination of omeprazole. Methods Strong chemiluminescence (CL) signal was found generated when omeprazole was injected into the reaction mixture of KMnO4 and luminol. The experimental conditions that affected the CL reaction were optimized by using FI mode. Results Under the optimum experimental conditions (150cm of mixing tubing, 0.15mol / L NaOH, 7.0 10-5mol / L luminol, 2.0 10-5mol / L KMnO4 and 2.8mL/min of flow rate), CL intensity was linearly related to the concentration within the range of 2.0 10-8-1.0 10-5g/mL. The detection limit was 3 10-9g/mL omeprazole, and the relative standard deviations (n = 11) were 1.0% and 2.6% for 1.0 10-7g/mL and 1.0 10-6g/mL omeprazole solut