Determination of plasma prothrombin time.docVIP

 PAGE \* MERGEFORMAT 9 Determination of plasma prothrombin time [Abstract] Objective measurement of prothrombin time and clinical significance of the operation, provide the basis for clinical treatment. Methods 6 studies conducted in our department in recent years, prothrombin time testing report. Conclusion PT determination must be standardized operation and reporting to correctly guide the clinical use of drugs. [Keywords:] Determination of prothrombin time Determination of plasma prothrombin time (prothrombin time, PT), is the extrinsic coagulation pathway in vitro all the conditions required for determination of plasma coagulation time, to reflect the exogenous factor is abnormal coagulation pathway, is the screening coagulation the most basic functions, one of the most commonly used test. 1. Principle In the plasma are, by adding a sufficient amount of tissue thromboplastin (tissue factor, TF) and the amount of calcium ions, can meet all the conditions of exogenous clotting. From adding calcium to the plasma clotting time required is called prothrombin time. PT reflects the length of the plasma prothrombin, fibrinogen and factor V, , X level. 2. Reagents (1) anticoagulants: 109 mmol / L sodium citrate solution (equivalent with two molecules of crystal water sodium citrate 32 g / L). (2) The thromboplastin reagent: commercial products, should be marked with ISI, lot number and expiration date. Lyophilized instructions specified by the solution should be reconstituted. (3) calcium chloride (CaCl2) solution: the concentration of 25 mmol / L (Currently, most products have thromboplastin reagents, buffers, pre-mixed with calcium chloride solution, and can not reprovision CaCl2 solution). (4) quality control materials (normal and abnormal control plasma): there is a product supply. 3. Instruments (1) Manual method: stopwatch, 36.5 ~ 38.5 to maintain a constant temperature water bath. (2)