Determination of immunochromatography sulfadiazine in animal derived food residues.docVIP

 PAGE \* MERGEFORMAT 12 Determination of immunochromatography sulfadiazine in animal derived food residues Author: Wang Liang Jin Xiue When Li Kui XIONG Ning Shi Ding-Ren Bi [Abstract] established in animal derived food sulfadiazine residues immunochromatographic rapid determination method. Sulfadiazine with colloidal gold-labeled monoclonal antibodies as a chromogenic agent, sulfadiazine competitor coated on cellulose nitrate membrane chromatography as a capture reagent, the use of competitive reaction model made of colloidal gold immunochromatographic test strip. The immunochromatographic test strip can be completed within 15min of sulfadiazine residues in the semi-quantitative detection. Reading of the system determine the sensitivity of 5ng/ml, the naked eye to determine the detection limit sensitivity of 10ng/ml, with other sulfa drugs without cross-reaction. The method in different food products of animal origin (chicken, eggs, chicken livers, pork, liver, milk, honey), the recoveries of added sulfadiazine 68.1% ~ 118.8% range. Animal of the test sample test results show that the method and ELISA, and HPLC with a good line rate. This method is high sensitivity, detection accuracy, simple, fast and has good prospects for the development and application. [Keywords:] colloidal gold; immune chromatography; sulfadiazine ABSTRACT An immunochromatographic assay (ICA) was developed for the detection of sulfadiazine in food products of animal origin. Based on the competitive reaction mechanism, the competitor of sulfadiazine was coupled with the nitrocellulose membranes acted as the capture reagent. Colloidal gold was coulped with monoclonal antibody against sulfadiazine served as the detection reagent. With this method, semi  quantitative detection of sulfadiazine can be completed in less than 15min. The sensitivity to sulfadiazine was 5ng/ml by strip reader and 10ng/ml by eye measurement, and no cross  reactivity with other sulfonamides. The recoveries w