Different areas on the determination of organic acids in Isatis.docVIP

 PAGE \* MERGEFORMAT 7 Different areas on the determination of organic acids in Isatis [Abstract] Objective To compare the different areas of organic acid content in Isatis. Methods Daqingye organic acids in Radix 3 main characteristics of UV absorption spectra, using Shimadzu UV-2401 UV spectrophotometer big different areas organic acids in leaf herbs and methodology were investigated. The results from different areas of organic acids in Isatis significant difference. Conclusion The method is simple, accurate, reproducible quantification of organic acids as Daqingye analysis. [Keywords:] Folium, organic acids, UV spectrophotometry Abstract: Objective To compare the content of organic acid in Folium Isatidis from differernt Habitats. Methods According to ultraviolet absorption atlases characteristics of the three main organic acid in Folium Isatidis, DSA UV-2401 was used to determinate the acidcontent of Folium Isatidis from differernt Habitats , and carried on to inspect the methodology. Results The difference of the acidcontent of Folium Isatidis from differernt Habitats is notable. Conclusion The method is simple, accurate and duplicated good, and can be used as quantitative analysis method of organic acid in Folium Isatidis. Keywords:: Folium Isatidis, organic acid, ultraviolet spectrophotometric method Salicylic acid, syringic acid and benzoic acid are the three kinds of Isatis common organic acids. For the determination of organic acids in Isatis methods have been reported related to [1], but these methods are more cumbersome. In this study, was determined by UV spectrophotometry, the results are more accurate for quantitative analysis of organic acids in Isatis reference method. An instrument and reagent Shimadzu UV-2401 UV spectrophotometer, the Swiss Mettler AE-100 millionth electronic analytical balance, HH-S-type water bath. Benzoic acid and salicylic acid reference substance (China Pharmaceutical and Biological Products, a