Different periods of losartan treatment of diabetic nephropathy Comparative Study.docVIP

 PAGE \* MERGEFORMAT 3 Different periods of losartan treatment of diabetic nephropathy Comparative Study Liu Gang, Guan Guangju △ , ZHAO Jing-Jie *, Fu Qin Yu, Li Xue-Gang, Wu Tao, Wen-Rong Zhu, Qi with the steel [Abstract] Objective To observe the occurrence of microalbuminuria before and after losartan on diabetic nephropathy and its mechanism. Methods unilateral nephrectomy in STZ diabetic rats were randomly divided into normal control group (NC), diabetic nephropathy (DN), losartan early treatment group (DL1) and losartan in the late treatment group (DL2). DL1 and DL2 in the diabetic model group were immediately or after the establishment of the first nine weeks since given losartan 20mg / (kg * d) were fed treatment were 8 weeks. The first 16 weeks, the observation of renal morphology and renal function changes, and using fluorescent real-time quantitative RT  PCR detection of renal TGF-β 1, CTGF, PAI-1 expression of cytokines, such as. Results DN control rats microalbuminuria (UAER) continued to rise; serum creatinine (SCr), index of kidney hypertrophy (KW / BW), mean glomerular volume (MGV), mesangial area ratio (FMA), as well as kidney TGF -β 1, CTGF, PAI-1 and FN mRNA expression were significantly higher, while creatinine clearance rate (CCr) has begun to fall. DL1 and DL2 group were significantly more improved, but no significant difference between the two groups of. Conclusion Losartan can cytokine by down-regulation of renal gene expression, play a role in renal protection; but microalbuminuria does not intervene before the appearance of microalbuminuria than in treatment with the advent of better. [Keywords:] diabetic nephropathy; transforming growth factor; connective tissue growth factor; plasminogen activator inhibitor -1; Losartan [Abstract] Objective To estimate the renoproctive effect of angiotensin receptor antagonists on DN by applying losartan before or after microalbuminuria.Methods 40 uninephrectomized male Wistar rats are rand