Different varieties of puerarin and puerarin Determination of total flavonoids.docVIP

 PAGE \* MERGEFORMAT 13 Different varieties of puerarin and puerarin Determination of total flavonoids Author: WANG Yan-Cai Duan Jin-ao Su Shu-Lan Hua Yongqing [Abstract] Objective To establish the different species of puerarin and puerarin method for the determination of total flavonoids. Methods RP-HPLC method using methanol - water (32:68) as mobile phase, detection wavelength of 250 nm measured puerarin content; ultraviolet spectrophotometry Cai ivy, kudzu of total flavonoids. The results raise the degree of puerarin peak separation, peak shape improvements; 0.204 ~ 2.04 μ g puerarin and the peak area within the linear relationship was good (r = 0.999 9), recovery was 98.7% (n = 5), precision RSD = 0.86 %. The concentration of total flavonoids 4.08 ~ 16.32 μ g / ml and the absorption within the linear relationship was good (r = 0.999 8). CONCLUSION The method is sensitive, accurate, simple, reproducible and can be used for traditional Chinese medicine herbs and Radix Pueraria quality control. [Keywords:] Cai Ge Ge Gefen prime root of total flavonoids by high performance liquid chromatography Abstract: ObjectiveTo study the determination method of puerarin and isoflavones in Pueraria lobata (Willd) Ohwi and Pueraria thomsonii Benth. MethodsThe separation was performed on RP  HPLC with a mobile phase of CH3OH-H2O (32:68 V / V) solution and the detection wavelength was 250 nm for the determination of Pueraria. UV-spectrophotometry for the determination of isoflavones.ResultsPuerarin and other peaks were separated well and the peak shape was presented well. There was a good linearity within the range of 0.204 to 2.04 μ g of Puerarin ( r = 0.999 9). The average recovery was 98.7% (n = 5). The RSD was 1.3%. The isoflavones had a good linearity within the range of 4.08 ~ 16.32 μ g / ml (r = 0.999 8). Conclusion This method is sensitive, accurate and reproducible. It can be used as the quality control method of Pueraria lobata and drug. Keywords:: Pueraria l