DIM combined with tumor necrosis factor-related apoptosis-inducing ligand inhibition of prostate cancer.docVIP

 PAGE \* MERGEFORMAT 11 DIM combined with tumor necrosis factor-related apoptosis-inducing ligand inhibition of prostate cancer Of: Kuangyou Lin Weng Xiaodong Chen Zhiyuan Liu Xiuheng Zhuheng Cheng Jiang waves [Abstract] Objective To investigate 3,3-b indole methane (DIM combined with tumor necrosis factor-related apoptosis-inducing ligand (TRAIL inhibition of prostate cancer cells. Methods Determination of CCK 8 DIM, TRAIL and TRAIL combined effect of DIM on the PC cell survival after 3 cells, while cells with the apoptosis rate by flow cytometry, Western blot the expression levels of caspase 3. Results of the treatment group compared with the control group could inhibit the PC 3 different degrees of cell proliferation and induced apoptosis, and upregulation of caspase 3, DIM and TRAIL combined with significantly enhanced induction of apoptosis. Conclusions and TRAIL combined with DIM can be significantly enhanced inhibitory effect of PC 3 cells and induction of apoptosis, the mechanism of apoptosis may be related increased caspase 3 expression. [Keywords:] prostate cancer, tumor necrosis factor related apoptosis inducing ligand, 3,3 b indole methane, apoptosis [Abstract] Objective To investigate the inhibitory effect of 3, 3 diindolylmethane (DIM) in combination with TNF related apoptosis inducing ligand (TRAIL) on the prostate cancer cells. Methods CCK 8 methods was used for detecting the survival rate of PC 3 cells treated with DIM, TRAIL, or DIM TRAIL. The apoptosis rate of PC 3 cells was tested by flow cytometry (FCM). The expression of caspase 3 was measured by Western blot. Results Compared with that of control group, the proliferation of PC 3 cells was inhibited and the apoptosis was induced differently in all treatment groups, and the expression of caspase 3 was up regulated. The combined use of DIM TRAIL induced apoptosis of PC 3 cells. Conclusions Combination use of DIM and TRAIL could obviously enhance the inhibitory effects and apoptos