Distribution of blood in patients with sepsis and antibiotic resistance of bacteria.docVIP

 PAGE \* MERGEFORMAT 8 Distribution of blood in patients with sepsis and antibiotic resistance of bacteria Sepsis is a common clinical infectious diseases, with acute onset, mortality is high. To understand the original bacterial sepsis and drug resistance characteristics and clinical medicine to improve the cure rate and reduce mortality are of great significance . We are from December 2007 to December 2009 inspection of the 540 cases during the clinical blood specimens, 111 strains of bacteria isolated from its composition in the blood samples were compared and drug resistance analysis, are reported below. 1 Materials and methods 1.1 The source of strain December 2007 to December 2009 self-hospital 540 cases of suspected sepsis, blood cultures of clinical specimens, 111 strains of bacteria isolated. 1.2 Methods Standard procedures for blood collection blood, were injected into the blood culture aerobic and anaerobic resin bottle, placed BACTEC9050 automated blood culture instrument for training, equipment positive alarm, remove the positive bottles, taken to smear the blood culture bottle and isolation and culture, bacterial growth came out, with the identification of susceptibility of bacteria Fosun company FOUTUNEIMS analysis system for bacterial identification and susceptibility testing. 1.3 The extended spectrum β-lactamase detection Of the bacteria Escherichia coli and Klebsiella, with ceftazidime / clavulanic acid and ceftazidime to detect extended spectrum β-lactamase inhibition of both the difference between the grate ring diametergt; = 5mm for the ultra-broad-spectrum β- lactamases positive. 1.4 Quality Control With ATCC25922, ATCC25923, ATCC27853 quality control strains for quality control. 2 Results 2.1 540 cases of blood culture samples than in the composition of bacteria 540 blood samples of bacteria were isolated from 111, the positive rate was 20.5%, and 72 strains of Gram-negative bacteria, acc