基因工程的发展历史1.分子生物学基础建立期.ppt

基因工程的工具及方法;限制酵素(restriction enzyme);連接酵素(ligase);載體(vector);載體之功能分類;載體之功能分類(continued);宿主(host);各種使用於基因工程之宿主特點比較;Expression of human insulin in E. coli;Bacterial production of human growth hormone;Bacterial production of human growth hormone (b);Production of a subunit vaccine in yeast;Salvage Pathway;Production of tissue plasminogen activator (tPA);Production of a monoclonal antibody (MAb);A bispecific antibody;基因工程之新應用;全球生技藥品市場及成長率;生技藥品市場分配份額;不同研發階段之生技藥品;紅血球生成素(Erythropoietin, EPO);紅血球生成素之臨床應用;EPO市場概況;干擾素(Interferons, IFN);干擾素之生產;干擾素之臨床應用;干擾素之市場分析;胰島素(Insulin);胰島素製造方法;胰島素市場分析;單株抗體(monoclonal antibody);單株抗體技術演進;單株抗體技術演進;老鼠單株抗體技術(Murine monoclonal antibodies);老鼠單株抗體商品化的限制因素;嵌合單株抗體;仿人類單株抗體;人類單株抗體;單株抗體之產品現況;血液因子;血液稀化劑;纖維消化酵素;血栓溶解劑;凝血蛋白質(clotting proteins);細胞集落刺激因子(colony stimulating factor, CSF);Granulocyte-macrophage colony stimulating factor (GM-CSF);Granulocyte colony stimulating factor (G-CSF);Macrophage colony stimulating factor (M-CSF);CSF市場研發狀況;生長賀爾蒙(Growth hormone);51;介白素(Interleukin);介白素-2 (Interleukin-2, IL-2);Biotechnology Drugs;Why Are Biotechnology Products Different From Simple Organic Chemicals?;Biotech Drugs;Manufacturing Processes Are Different;Biotechnology Drugs;Medicinal Products Derived From Recombinant DNA;Five Features For a Biotechnology Drug;Five Features For a Biotechnology Drug1. Expression System: Vector + Host;Expression System: Vector Construction + Host;Five Features For a Biotechnology Drug2. The Production System;Five Features For a Biotechnology Drug3. The Purification System;Five Features For a Biotechnology Drug4. Nature of Active Product;Five Features For a Biotechnology Drug5. Pharmaceutical Formulation and Presentation;Biotechnology Drugs;Process Flow-Chart;A Cell Bank;The Master Cell Bank (MCB);The Manufacturer Working Cell Bank (MWCB);The Late Expanded Cell Bank (LECB);Cell Bank System;Cell Bank System;Process Flow-Chart;Process Flow-Chart;Process Flow-Chart;Purification: Potential Contaminants;Purification: Potential Co