Electric drive and the cation exchange membrane in the concentration of DNA capture.docVIP

 PAGE \* MERGEFORMAT 13 Electric drive and the cation exchange membrane in the concentration of DNA capture Of: Huanghua Bin Zhang Wenhua Hu Jia Zhuang Zhi House * Wang Yang Zhaoyong as [Abstract] based on characteristics of electric drive with the choice of ion exchange membrane permeability to construct new, no damage to the DNA (DNA capture enrichment devices. To λ-DNA as a model compound to explore its rich self-assembly device set of features optimized experimental conditions, to concentrate the DNA gel electrophoresis to examine whether the variability, and the polymerase chain reaction to demonstrate the method of recovery of DNA available for further biological analysis. The results show that: the device in low voltage conditions can be captured without damage to DNA, concentrated liquid can be used for further biological analysis, DNA of the enrichment factor of 62 times the return rate of 46%. The method is simple, avoid using large amounts of organic reagent, with the miniaturization of biological chip combined with the DNA extraction, purification and concentration areas of greater use of space. [Keywords:] DNA capture, electric drive, ion exchange membrane, concentration Abstract Based on the selective permeability of cationic exchange membrane and the characteristic of electrical driven, a new and non-damage apparatus that can be used to separate compounds with different electric property was built to capture and enrich DNA.λ-DNA, as a model compound, was used to study the characteristic of capturing and enriching DNA in this device.Factors that might affect the efficiency such as voltage and flow speed were optimized and gel electrophoresis was done to study if DNA was denaturation, PCR was done to prove the concentration can be used to further bioanalysis.Results were got: the apparatus could used to capture and enrich DNA scathelessly under the condition of low-voltage, the concentrate multiple was 62, and the recovery efficiency wa