ELISA reagents flower board the problem from occurring and analysis of.docVIP

 PAGE \* MERGEFORMAT 5 ELISA reagents ‘flower board’ the problem from occurring and analysis of [Keywords:] ELISA reagents flower plate problem Using the ELISA reagents for blood HBsAg, HCV, HIV and syphilis, and other inspection process, often find gaps and negative, positive control, as well as internal quality control hole (Ministry of Health stops Center, HBsAg 0.5ng, 1ng; HCV, HIV, syphilis are 2NCU) the results of normal, while blood samples OD value of the hole higher than that (most of OD values ≥ 0.070). A high rate of positive specimens, each plate except the control hole, the positive and sometimes as many as up to 10 holes, while the majority of true positive samples weak positive results have emerged. This is the test described in the ‘flower board’ issues. Of this phenomenon on the normal test results to determine the impact of causing a great deal. I During the trial, the response to this ‘flower board’ phenomenon a careful analysis and judgments. Summarized below. 1 specimens, reagents, equipment 1.1 specimen in 2008 from January to December unpaid blood donors in blood samples. 1.2 reagent HBsAg, HCV, HIV, syphilis, ELISA reagents are reagents of different manufacturers. 1.3 Instrument LD4-2 centrifuge, BID RAD1575-type plate washer, BID RAD550 microplate reader. 2 Methods Unpaid blood donors of all blood samples, in accordance with the various manufacturers instructions provided by the ELISA reagents for HBsAg, HCV, HIV, syphilis test item operation. All reagents were used within the validity period. 3 Results Reagents used by different manufacturers occasionally occur ‘flower board’ issues. 4 Analysis In all specimens tested in the process and processing equipment used in the sample are in the process of normal circumstances, the steps are in accordance with the requirements in the specification of various reagents, excluding experimental apparatus and steps used in the experimental results of the impact, reagent box blank, negat