Embryonic mouse kidney development in neuronal nitric oxide synthase in quantitative analysis of.docVIP

 PAGE \* MERGEFORMAT 11 Embryonic mouse kidney development in neuronal nitric oxide synthase in quantitative analysis of Author: Mu Changzheng Wang Xiaomei Tao Shi Ying Liu Xia package Cuifen [Abstract] Objective: To investigate the neuronal nitric oxide synthase in embryonic kidney development in mice in different periods of pregnancy meaning. Methods: Healthy adult Kunming mice, male and female ratio of 3:1 according to the same nest feeding, use of check off vaginal suppository of the method to determine the female time to pregnancy. Select the age of 12 days embryo (ED12), 14 Tian (ED14), 16 Tian (ED16), 18 Tian (ED18) of fetal and birth (PD0) offspring divided into five groups, each group of 6 female mouse, Each female mouse obtained 2 fetal or neonatal rat, Cesarean section to take the kidney, using Western blot technique (Western blot) and the optical density analysis of the system separately for each group of fetal or neonatal rat kidney for quantitative detection of nNOS. Results: Western blot and optical density analysis showed that, ED14 group nNOS content in the kidney at least, and then gradually increased, PD0 group of renal nNOS the most abundant. Conclusion: The embryonic mouse kidney nNOS in the embryonic day 14 showed positive expression levels gradually increased after birth, when the highest levels, indicating that nNOS in the embryonic mouse kidney development plays an important role in the early stages. [Keywords:] neuronal nitric oxide synthase; embryonic mouse; kidney Of nitric oxide synthase (Nitric Oxide Synthase, NOS) is the nitric oxide (NO) synthesis rate-limiting enzyme, is an important part of regulating NO. NO produced by NOS, is a messenger molecule can be used as free radical gas [1], can activate soluble guanylyl cyclase, by generating cGMP play a role. NOS can be divided into endothelial (endothelial NOS, eNOS), neuronal (neuronal NOS, nNOS) and inducible (inducible NOS, iNOS) 3 categories. NO in renal hemodynamics and