Explant culture of rabbit aortic smooth muscle cells.docVIP

 PAGE \* MERGEFORMAT 10 Explant culture of rabbit aortic smooth muscle cells Of: Wang Qingqing, Li Weihong, Zhijie Chang, Jiao Jinju [Abstract] Objective cultured rabbit aortic smooth muscle cells of the way for the cause of cardiovascular disease provide a useful mechanism in vitro model. Methods adherent tissue, dry method of reverse rabbit aortic smooth muscle cells cultured and passaged. Inverted microscope The following were observed and identified by immunocytochemistry. Results of vascular smooth muscle cells were successfully cultured and passaged. inverted microscope, cells showed the typical “peak - valley” shape. immunocytochemistry showed brown, showed positive expression of cytoplasmic seen in the longitudinal rows of filaments. Conclusion tissue adherent, dry method of successful reversal of rabbit aortic smooth muscle cells in primary culture, with a simple, short training period, the purity of advantages, is a good cardiovascular disease in vitro research model. [Keywords:] vascular smooth muscle cells; primary culture; rabbit Abstrct: Objective To discuss the method for primary culture of rabbit arterial smooth muscle cells.To provide useful vitro model for the etiopathogenisis of cardiac vascular diseases. Methods Rabbit arterial smooth muscle cells were cultured with tissue explants adherent and back to roll depletive method and could be passaged.The form of rabbit arterial smooth muscle cells were observed under inverted phase contrast microscope and were identified with the method of immunocytochemistry. Results Rabbit arterial smooth muscle cells were cultured successfully with tissue explants adherent and back to roll depletive method and could be passaged . Under inverted phase contrast microscope, the growth of the cells showed a typical hill-and-vally pattern.After immunocytochemistry staining, all the cells cytoplasm were stained and positive.Lots of stained buffy actin filament bundles were observed in cell cytoplasm. Con