Ginkgo biloba extract on PC12 cells in 6-OHDA induced oxidative stress.docVIP

 PAGE \* MERGEFORMAT 14 Ginkgo biloba extract on PC12 cells in 6-OHDA induced oxidative stress [Abstract] Objective To investigate the Ginkgo biloba extract (Ginkgo biloba extract, GBE) on the 6 - hydroxydopamine (6-hydroxydopamine, 6-OHDA)-induced oxidative stress in PC12 cells and its mechanism of protection. Methods pre-treated with different doses of GBE PC12 cells cultured in vitro after adding 6-OHDA induced dopaminergic neuronal injury models. PC12 cells by MTT viability assay; Determination of cell catalase (CAT), glutathione peroxidase ( GSH-Px), total superoxide dismutase (T-SOD) activity and total antioxidant capacity (T-AOC) level; thiobarbituric method cells MDA (MDA) content. Results 100 mol / L 6-OHDA treatment of PC12 cells 24 h, cell viability was significantly lower than normal control group (P lt;0.01), CAT, GSH-Px, T-SOD activity and T-AOC levels were significantly lower (P lt;0.05) , MDA was significantly higher (P lt;0.05); with the model group, different doses of GBE pretreated group gradually increased PC12 cell viability, CAT, GSH-Px, T-SOD activity and T-AOC levels rose significantly higher (P lt;0.05), MDA content was significantly decreased (P lt;0.05). Conclusion GBE on 6-OHDA-induced oxidative stress in PC12 cells inhibited increased CAT, GSH-Px, T-SOD activity and T-AOC the level of its antioxidant effect may be one of the mechanisms. [Keywords:] Ginkgo biloba extract; 6 - OHDA; PC12 cells; oxidative stress Abstract: Objective To explore the protective effects and the causal mechanism of Ginkgo biloba extract (GBE) on the oxidative stress induced by 6-hydroxydopamine (6-OHDA) in PC12 cells. Methods Dopaminergic neuronal injury model was induced by addition of 6-OHDA into PC12 cells cultured in vitro and pretreated with different doses of GBE. Cell viability, the levels of four antioxidative indexes [including total superoxid dismutase (T-SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and total antioxidant capacity