Ginkgo biloba extract on rat retinal ganglion cells in the protective effect.docVIP

 PAGE \* MERGEFORMAT 10 Ginkgo biloba extract on rat retinal ganglion cells in the protective effect [Abstract] Objective To study the acute ocular hypertension in ischemia-reperfusion model of Ginkgo biloba extract on the protection of retinal ganglion cells. Methods experimental SD rats were divided into 3 groups 30 were negative control group, positive control group , treatment group, n = 10. right eye was injured rat eyes, respectively, the formation of anterior chamber puncture perfusion to maintain the intraocular pressure of 110 mmHg 60 min, left without making damage, as normal controls. negative control group after injury 2 h and then were administered daily intraperitoneal saline 5 mL / kg. by this administration method, the positive control group received 1% Erigeron 5 mL / kg, treatment group received 1% of Ginkgo biloba extract 5 mL / kg, the duration of 28 d. experimental animals 5 d before euthanasia with 3% fluorogold double superior colliculus injection, retrograde labeling of retinal ganglion cells. Do retinal directional stretched from the center of the optic nerve 2 mm left and right upper and lower photographs. Using particle analysis software to do section of color cell count, calculate the survival rate of ganglion cells in each group and statistical analysis. Results negative control, positive control group, treatment group ganglion cell survival rates were 61.46%, 72.73%, 76.20%. Medication group and negative control group of ganglion cells there were significant differences in survival (P lt;0.05), treatment group and positive control group had no significant difference (Pgt; 0.05), positive control group and negative control group was significant difference between (P lt;0.05). Conclusions In acute ischemia and reperfusion model of high intraocular pressure, the Ginkgo biloba extract can effective protection of retinal ganglion cells, and the positive control fleabane comparison, the effect of protecting both close to the ganglio