Ginkgo biloba extract on the rabbit retina after optic nerve crush protective effect of retinal ganglion cells.docVIP

 PAGE \* MERGEFORMAT 12 Ginkgo biloba extract on the rabbit retina after optic nerve crush protective effect of retinal ganglion cells OF: Joe Feng, Liu Yongxia, Liu Jinhua, Jiang Liping, as Bonaventure [Abstract] Objective: To study the optic nerve after injury EGb761 on rabbit retinal ganglion cells (RGC) in rats. Methods: 24 rabbits the right eye as the experimental group, left as a control group, production of optic nerve injury model. Right retrobulbar injection of EGb761 (60mg/kg), the capacity of the left eye after the injection of balanced salt solution such as BSS. 4,7,14 d after injury, drawn, applied pathological image analyzer counts RGC, and the myelin basic protein (MBP) in the immunohistochemical staining. Results: after injury 3 ~ 14d, the two groups decreased the number of RGC, the difference was statistically significant (P lt;0.01), the experimental group was significantly higher than the number of RGC saline group, the difference was statistically significant (P lt; 0.01), both groups after injury, positive expression of MBP, BSS group showed strong positive expression of the experimental group decreased MBP, 14d expression was weakly positive, BSS group expression is also weakened with time, but when the expression was still significantly 14d , the experimental group at different time points were lower than the positive RGC BSS group differences were statistically significant (P lt;0.01. Conclusion: EGb761 after optic nerve injuries can reduce the content of MBP, raise the number of RGC survival. [Keywords:] EGb761, retinal ganglion cells, optic nerve injury, rabbits, myelin basic protein Abstract ?r AIM: To observe the protective effect of EGb761 on retinal ganglion cells (RGC) in rabbits with optic nerve crush injury by myelin basic protein (MBP) in rabbits’ retina.METHODS: Twenty four rabbits were divided into experimental (right eyeand control (left eye) groups. The rabbits’ optic nerves were crushed by mini vascular nip.