Ginkgo biloba extract-induced rat aortic smooth muscle cells in the expression of heme oxygenase -1 experimental study.docVIP

 PAGE \* MERGEFORMAT 12 Ginkgo biloba extract-induced rat aortic smooth muscle cells in the expression of heme oxygenase -1 experimental study Author: Wang Hongxia, Yang Xiaoyu, WEI Zongde, Huang Yi [Abstract] Objective To investigate the Ginkgo biloba extract (EGb)-induced rat aortic smooth muscle cells (SMC) in the heme oxygenase -1 (HO-1) expression in dose-effect relationship and to explore EGb-induced HO-1 expression in The molecular mechanism. Method of recovery and subcultured rat aortic SMC, group experiments, respectively 0,50,100,200,400 μ g / ml 5 different concentrations of EGb, as well as 200 μ g / ml EGb plus actinomycin D (ActD) were incubated SMC 8 h, Western blot to detect HO-1 protein, to HO-1 band gray-scale value represents the amount of HO-1 expression. The results of the control group without EGb in HO-1 protein expression in only trace basis, plus with EGb each group HO-1 protein expression with EGb concentration increases (P amp;lt;0.05), in the EGb concentration of 200 μ g / ml, reached its peak, and then fell. Added by ActD could significantly inhibit the EGb-induced HO-1 protein expression (P amp;lt;0.05). Conclusion EGb could be induced in rat aortic SMC in the HO-1 expression, and this expression within a certain range with the dose-effect relationship; EGb-induced HO-1 gene expression depends on the re-transcription. [Keywords:] Ginkgo biloba extract in rat aortic smooth muscle cell heme oxygenase -1 Abstract: ObjectiveTo explore the dose-effect relationship and the molecular mechanism of the heme oxygenase-1 (HO-1) expression induced by the extract of Gingko biloba (EGb) in rat aorta smooth muscle cells (SMC). MethodsThe SMC was revived, passage cultivated and divided into different groups.The cells in each group were incubated with 5 concentrations of EGb such as 0,50,100,200,400 μ g / ml and EGb 200 μ g / ml ActD4 μ m respectively. The incubation lasted for 8 hours , then Western blot was applied to analyze HO-1 protein expr