Ginkgolide B on cholesterol and apolipoprotein E4 injury in hippocampal neurons of cholesterol metabolism.docVIP

 PAGE \* MERGEFORMAT 13 Ginkgolide B on cholesterol and apolipoprotein E4 injury in hippocampal neurons of cholesterol metabolism Of: Jiangxi Juan Guomao Juan Su Jinling Wang Qiang Xue Jie Zhang Ruifeng Ma Yiwen TRADITIONAL [Abstract] Objective ginkgolide B on the high cholesterol and apolipoprotein E4 (ApoE4) injury in hippocampal neurons of cholesterol metabolism. Methods 40 g / ml cholesterol and 30 g / ml ApoE4 neurons co-processing, simulation of high Cholesterol cell injury model. ginkgolide B after the intervention of this model, the real-time quantitative polymerase chain reaction (RT PCR) method of low density lipoprotein receptor protein were measured (LRP) 1 mRNA, ATP binding cassette rotor A ( ABCA) mRNA and endoplasmic reticulum acetyl cholesterol acyltransferase (ACAT) mRNA expression were detected by Western blot LRP1, ABCA1, and ACAT1 protein. Results of model group increased LRP1 mRNA expression of ABCA1 and the expression of ACAT1 mRNA upward trend, but compared with the control group, no significant difference. ginkgolide B can increase ACAT1 mRNA expression, compared with model group were significantly different. the factor protein expression and mRNA expression of consistent trends, and model group with There were significant differences between groups. Conclusion of cholesterol and combined effects of ApoE4 neurons cholesterol intake, conversion of cellular cholesterol and cholesterol excretion. ginkgolide B on the high cholesterol cholesterol metabolism of neurons under a certain degree of regulation. [Keywords:] cholesterol; ginkgolide B; low density lipoprotein receptor-related protein 1; ATP binding cassette transporter protein 1; acyl cholesterol acyltransferase 1 A large number of studies have shown that abnormal brain cholesterol metabolism in Alzheimer’s disease (AD) play an important role in the pathogenesis, particularly in the apolipoprotein E4 (ApoE4) isoform dominant groups, especially the role of (1). The blood br