Ginkgo biloba microwave DPPH Radical Scavenging Technology.docVIP

 PAGE \* MERGEFORMAT 12 Ginkgo biloba microwave DPPH Radical Scavenging Technology [Abstract] Objective To study the antioxidant activity of Ginkgo biloba in the best extraction of material. Methods of microwave-assisted extraction of Ginkgo biloba extract antioxidant to remove the IC50 value of DPPH free radical activity of tracking targets, using orthogonal pairs of extraction conditions were optimized. Results The optimum extraction process as follows: microwave irradiation time 20 min, ethanol volume fraction of 70%, solid-liquid ratio 1:20 (g: ml), extraction temperature 50 ℃ . Click here to optimize the conditions of the conclusions extracted, with an average IC50 value of 1.29 μ g / ml, ginkgo flavonoids yield could reach 3.1%. [Keywords:] orthogonal microwave extraction of Ginkgo biloba antioxidant Abstract: ObjectiveTo study the optimum extraction process of antioxidative substances from Ginkgo biloba leaves. MethodsMicrowave assisted extraction (MAE) was used to extract antioxidative substances from Ginkgo biloba leaves. The extraction conditions of antioxidative substances from Ginkgo biloba leaves were studied by orthorhombic analysis and IC50 of scavenging DPPH free radical was used as the activity monitoring parameter.ResultsThe optimum extraction conditions were: 20 min, extraction time, 70% ethanol at 50 ℃ , and a material / solvent ratio of 1:20 (g: ml). Conclusion Under this condition, IC50 of scavenging DPPH free radical of antioxidative substances from Ginkgo biloba leaves is 1.29 μ g / ml while the yield of flavonoids of Ginkgo biloba leaves is 3.1%. Keywords:: Orthogonal test method; Microwave assisted extraction; Ginkgo biloba leaves; Antioxidative substance The past 20 years, reactive oxygen and free radicals in modern life science research hot spots, evaluation and selection have strong antioxidant activity of natural resources has become a biology, medicine and food science of the new trend. Ginkgo biloba tree species endemic to C