Glucocorticoid receptor expression in pterygium and its significance.docVIP

 PAGE \* MERGEFORMAT 2 Glucocorticoid receptor expression in pterygium and its significance Author: Qi Chao Xiu, Wang Xiaoli, Li Qi root, Juan Deng, YE Hui [Abstract] [Objective] To examine the glucocorticoid receptor-α , β (GR-α , GR-β ) in pterygium tissue, to explore its role in the pathogenesis of pterygium. [Method] 39 patients with pterygium organizations (including the initial issuance of 30 cases of recurrence in 9 cases), by fluorescence quantitative RT-PCR quantitative detection of GR-α , β mRNA expression, application of Western blotting analysis of the corresponding protein expression technology, while technical analysis by immunohistochemistry protein cellular localization. In addition, take nine cases of normal bulbar conjunctiva as controls. [Results] pterygium and normal bulbar conjunctiva tissue were expressed GR-? Zhuo mRNA, no expression of GR-β mRNA. One pterygium (39 cases) in the GR-? Zhuo mRNA (* 103 copies / μ g) for 10 ± 4, significantly higher than normal bulbar conjunctiva of 2.2 ± 0.3 (P amp;lt;0.01), significant by statistical test differences. However, 30 cases of pterygium and 9 newly diagnosed patients with recurrent pterygium in the GR-α mRNA expression was 10 ± 4 and 10 ± 5, no significant difference between the two (Pamp;gt; 0.05). Western blotting analysis showed that GR-? Zhuo protein expression in pterygium was significantly higher than normal ball conjunctiva (P amp;lt;0.01). Immunohistochemistry showed that GR-? Zhuo protein located in epithelial cells, pterygium The positive expression rate was 87.2%, significantly higher than the normal conjunctival group (positive expression rate of 22.2%, P