Glutamate transporter inhibitor DHK inhibited protective effect of ischemic preconditioning.docVIP

 PAGE \* MERGEFORMAT 6 Glutamate transporter inhibitor DHK inhibited protective effect of ischemic preconditioning [Abstract] Objective: To observe the glutamate transporter GLT1 inhibitor dihydro-Cain salts (DHK) on cerebral ischemic preconditioning (CIP)-induced brain ischemic tolerance. Methods: Using four-vessel occlusion method produced large rat model of global cerebral ischemia, the right lateral ventricle injection of DHK. thionin staining of brain tissue section was observed in hippocampal CA1 pyramidal neurons in delayed death (DND) level to determine the histological grade. Results: sham-operated group and the CIP group hippocampal CA1 area was no obvious DND; injury in ischemic hippocampal CA1 area significantly DND; CIP injury in ischemic hippocampal CA1 area DND is not obvious; DHK CIP injury in ischemic groups, DHK blocked CIP pairs hippocampal CA1 pyramidal neurons in the protective effect. quantitative analysis found that, DHK CIP traumatic injury in ischemic group than in the CIP the protective effects of ischemic group significantly reduced. Conclusion: DHK inhibited the CIP-induced brain ischemic tolerance. [Keywords:] Cerebral ischemic preconditioning; dihydro-Cain salt; hippocampus 0 Introduction Cerebral ischemic preconditioning (cerebral ischemic preconditioning, CIP)-induced brain ischemic tolerance (brain ischemic tolerance, BIT) by the Japanese scholar Kitagawa et al [1] was first discovered in 1990. The discovery of this phenomenon for the study of the body source nature of anti-ischemic mechanism of injury provides a new idea, so become a focus of concern, BIT-induced mechanism has been articulated. Past research on the mechanism of BIT more focused on their anti-ischemic neuronal injury mechanisms, The CIP is induced by glial cells and the BIT has not been reported. This study aimed to investigate astrocyte glutamate transporter GLT1 is involved in the CIP-induced BIT. 1 Materials and methods 1.1 Materials Male Wistar rats (