Gly salt deoxycholate normal liver cells induced apoptosis in HL7702 and its mechanism.docVIP

 PAGE \* MERGEFORMAT 13 Gly salt deoxycholate normal liver cells induced apoptosis in HL7702 and its mechanism Study: Min Chuan Liang, Hong Hua, Huang Wenfang, Yang Long, Raoshao Qin, Deng Jun [Abstract] Objective To observe Gly salt deoxycholate (glycodeoxycholate, GCDC) on human normal liver cell line HL 7702 apoptosis, and to explore its mechanism of apoptosis. Methods final concentrations of 100,150,200,250 mol / L The GCDC treatment HL 7702 cells 24h, optical microscopy GCDC on the HL 7702 the cell morphology, AnnexinV FITC / PI double staining HL 7702 the apoptosis rate, measured with the fluorescent indicator Fluo 3/AM intracellular calcium HL 7702 ion concentration, RT PCR determination of Bcl 2, Bax gene mRNA. Results final concentration of 150mol / L of GCDC HL 7702 cells 24h after treatment, cells showed typical apoptotic morphological changes, 100,150,200,250 mol / L, HL 7702 GCDC could induce apoptosis in a dose-dependent, apoptotic rates were (13.16 + -2.9)% (t = 6.41), (20.3 + -3.0)% (t = 10.22), (25.02 + -2.1)% (t = 18.11), (45.02 + -3.5)% (t = 28.89), compared with the control group (2.2 + -0.6)% was significantly higher (P lt;0.05), GCDC cells can HL 7702 calcium concentration increased in a dose-dependent, GCDC inside the cells decreased expression of Bcl 2 mRNA, Bax mRNA expression increased. Conclusion GCDC HL 7702 may be by increasing the concentration of intracellular calcium, and further reduced Bcl 2, increased Bax expression levels to induce apoptosis. [Keywords:] Gly salt deoxycholate, apoptosis, calcium, Bcl 2, Bax, liver cells ABSTRACT: Objective To investigate the apoptosis of cultured normal human hepatocyte HL 7702 cells induced by glycodeoxycholate (GCDC) and to explore its possible mechanism. Methods HL 7702 cells were incubated with various concentrations (100, 150, 200 and 250mol / L) of GCDC . The changes of cellular morphology were observed under optical microscope. The apoptosis rate of HL 7702 was determined b