Gold standard method and the method used in clinical test titer syphilis Effect_0.docVIP

 PAGE \* MERGEFORMAT 8 Gold standard method and the method used in clinical test titer syphilis Effect [Abstract] Objective To investigate the gold standard method and the titer assay TRUST Treponema pallidum. Methods using enzyme-linked immunosorbent assay, toluidine red titer method (TRUST) 147 cases of clinical samples of primary screening test, and screening 49 sera were positive for gold standard method plum and drug testing spirochetes. Results ELISA titer method (TRUST) test was positive in 49 cases with samples positive for gold standard method, 46 cases of both the coincidence rate 93.88 %. Conclusion gold standard method and (TRUST) titer method, the sensitivity, specificity was no significant difference (Pgt; 0.05), syphilis can be jointly used in clinical testing, to improve detection rate. [Keywords:] syphilis; antibody; gold standard method; titer method Large number of clinical data and the epidemiological survey, the incidence of syphilis in China has a rising trend year by year [1 2]. Therefore, the development of a simple, fast, high sensitivity and specificity of both screening method for the prevention of the disease, treatment and screening of a large number of blood donors is undoubtedly of great significance. For those who serological screening test is an important basis for diagnosis of syphilis in syphilis diagnosis, treatment and research has very important significance. Therefore, the Court will be gold standard method, TRUST titer method was applied to the detection of syphilis, TPPA is recognized as a better method for confirmation of syphilis antibodies, so the samples were tested, TPPA results were compared in order to observe the consistency of the results, now the analysis reported below. 1 Materials and Methods 1.1 General Information Units of the group were selected in April 2006 of 143 cases since the first of the primary screening test clinical samples, and then the results of screening 49 patients with pos