Gold standard method of hepatitis B surface antigen detection methodology of.docVIP

 PAGE \* MERGEFORMAT 7 Gold standard method of hepatitis B surface antigen detection methodology of [Abstract] from the sensitivity, specificity, accuracy and stability of four pairs of gold-labeled hepatitis B surface antigen detection method to explore the methodology and the respective gold standard method and enzyme immunoassay of 1200 cases of physical examination and clinical samples testing, found that gold-standard method for the detection of hepatitis B surface antigen specificity, high sensitivity, and enzyme immunoassay in line with the rate of 99.0%, and no special equipment, simple and fast, with a wide range of application value. [Keywords:] gold standard method; hepatitis B surface antigen Of hepatitis B surface antigen (HBsAg) are hepatitis B virus coat protein in the main component of the positive sign of hepatitis B virus infection, is to check the hepatitis B serum markers in the more important one [1]. Developed in recent years the gold standard to detect HBsAg, with the traditional enzyme immunoassay (EIA), compared with a simple, rapid, accurate, and does not require equipment, etc., can meet the clinical needs of the emergency quickly produce results, and more suitable for the use of in the epidemiological investigation, a very broad application prospects. 1 Materials and methods 1.1 Materials (1) gold-standard test paper: on Haihong Tai Biotechnology Development Co., Ltd. products. (2) HBsAg sensitivity of standard reference materials: China’s pharmaceutical products and biological products. (3) HBsAg enzyme immunoassay diagnostic kits, HBsAg, HBcAg, HBeAg-positive control sera: China joint ventures Shanghai Industrial Division Biotechnology Co., Ltd. products. (4) of serum samples: 1,200 medical and clinical serum samples, of which 172 patients with hepatitis B, healthy human serum 1028 were. 1.2 Method (1) 5ng/ml standard reference materials were diluted with saline into 4ng/ml, 3ng/ml, 2ng/ml, 1ng/ml several concentrations, depi