Gonadotropin-releasing hormone agonist and combined with cisplatin on ovarian cancer in nude mice the effects of chemotherapy and molecular mechanisms of.docVIP

 PAGE \* MERGEFORMAT 21 Gonadotropin-releasing hormone agonist and combined with cisplatin on ovarian cancer in nude mice the effects of chemotherapy and molecular mechanisms of Of: Weng Hui M, Wang Yi Feng, Lin Qiongyan [Abstract] Objective To study alone releasing hormone agonist (gonadotropin-releasing hormone agonist, GnRHa) and with cisplatin in ovarian cancer in nude mice the effects of chemotherapy and its molecular mechanism. Methods 24 nude mice peritoneal poorly differentiated ovarian serous adenocarcinoma cell line OVCAR-3, into the tumor were randomly divided into 4 groups, namely the control group (NS), cisplatin, GnRHa group (GnRHa + NS), combined treatment group (GnRHa + cisplatin). stripping tumor weight, tumor tissues were detected by electron microscopy in cell apoptosis was detected by immunohistochemistry in tumor tissues ki67, NFkb and caspase-3 expression. Results In the control group was not seen in apoptotic cells and apoptotic bodies; cisplatin with nuclear pyknosis edge set mainly in the early apoptotic cells; GnRHa + NS group showed cell apoptosis and necrosis dissolved; combined treatment group in the late apoptotic cells based. tumor weight and ki67 score: the control group were (0.149 + -0.072) g, (9.533 ± 2.380); cisplatin group (0.034 + -0.010) g, (6.283 + -0.491); GnRHa group (0.116 + -0.039) g, (8.733 + -0.531); combined treatment group (0.026 + -0.024) g, (7.70 ± 0.745). statistics, cisplatin group and combination group were tumor weight and the control group ki67 compared, the difference was significant (P lt;0.05); the GnRHa group and control group comparison of tumor weight and ki67, cisplatin group and the combined group the tumor weight and ki67 There was no significant difference (Pgt; 0.05). cisplatin group and combination group and ki67 tumor weight was significantly lower than the other two groups, the difference was significant (P lt;0.05); compared with the control group, DDP group, GnRHa group, reduced expre