Grifola frondosa polysaccharide synergistic anti-tumor effect of chemotherapy and its molecular mechanism of.docVIP

 PAGE \* MERGEFORMAT 22 Grifola frondosa polysaccharide synergistic anti-tumor effect of chemotherapy and its molecular mechanism of Author: Lu Zhenyu 1, Po-Hong 2, Furong Jie 3, Han White 3Δ 【Abstract】 Objective To investigate the anti-tumor polysaccharide from Grifola frondosa β cells, the role and concurrent chemotherapy drugs to increase their anti-tumor cells. Through the study of p53 and MDM2 discovered the molecular mechanism of synergy between the two. Methods CCK-8 was determined by β Grifola frondosa polysaccharide synergistic with chemotherapy drugs to treat the HCT116 cell survival rate after (cell survival rate, CSR); with the PCR method was determined by p53, MDM2 gene expression. Results (1) β polysaccharide from Grifola frondosa on tumor cells is higher than the direct killing effect of Ganoderma lucidum polysaccharides. (2) Maitake Polysaccharide β concurrent chemotherapy drugs to enhance their tumor cells in vitro. (3), Grifola frondosa polysaccharide β synergistic molecular mechanism of chemotherapeutic drugs and inhibit the tumor suppressor gene p53 antagonist MDM2 gene expression. Conclusion Grifola frondosa polysaccharide β has a direct anti-tumor effect and synergistic anti-tumor effects of chemical drugs. Keywords: Grifola frondosa; chemotherapy drugs; p53; MDM2 【Abstract】 Objective The efficiency of anticancer therapy is limited by drug reistance.Recent studies suggest that a polysaccharide extracted from Grifola frondosa is able to kill cancer cells in vivo and in vitro.However, the molecular basis for the polysaccharide from Grifola frondosa synergistic effects with cytotoxic drugs has to be well defined.Methods HCT116 cell survival rate were monitoued by CCK-8 kit.p53 and MDM2 gene expression were checked by semiquantitative PCR analysis of cellular RNA.Results Here we showed the polysaccharide from Grifola frondosa had more great anti -cancer effect than shell-broken spores of G. lucidum in HCT116 cell