Green Tea on Rat Vascular Calcification.docVIP

 PAGE \* MERGEFORMAT 15 Green Tea on Rat Vascular Calcification Of: Xu Hua, Wan set, Xiao-Ming Yang, Xiao-Ling Yang, Li Guizhong, Wang Tian, #8203;#8203;Wu Hai, Cao [Abstract] Objective To observe the consumption of green tea on vascular calcification and its possible mechanism. Methods gavage with intramuscular vitamin D3 and nicotine-induced rat model of vascular calcification. Were divided into control group, calcium group, low dose and high dose of green tea intervention group. Von Kossa staining, atomic absorption spectrometry determination of calcium content of radioimmunoassay in plasma and blood osteocalcin (osteocalcin, OC), angiotensin (angiotensin II, Ang ) and alkaline phosphatase (alkaline phosphatase, ALP) activity. Results of vascular calcification in rats, staining smooth muscle cells and intercellular matrix has a large number of black particles together with the control group, aortic calcium content, ALP activity, OC, and Ang levels were significantly increased by 50 g / L and 100 g / L green tea can inhibit vascular calcification in rats during, and high-dose effect was stronger than the low dose group compared with the calcification of vascular tissue calcification index decreased, and aortic Ang levels also reduced. Conclusion drinking green tea may be partially plasma and vascular tissue by antagonizing the local effect of Ang system suppression caused the role of vascular calcification. [Keywords:] Green tea angiotensin vascular calcification Abstract: Objective To explore the possible mechanism which was involved in the regulatory action of green tea on vascular calcification. Methods Rat vascular calcific model was made by vitamin D3 intramuscular injection and oral nicotine. Rats were divided into four groups: the control, the calcification , lower and higher dosage green tea groups. The total aorta Ca2 + content in aorta was measured by atom absorptive spectrophotography, the alkaline phosphatase (ALP) activity in pla