GPCgas chromatography mass spectrometry determination of pesticide residues in honeysuckle components 33.docVIP

 PAGE \* MERGEFORMAT 14 GPCgas chromatography mass spectrometry determination of pesticide residues in honeysuckle components 33 Of: Zhuangwan E Bow Zhen-Bin Jiang Lei Chen Dengyun leaf [Abstract] established a gel permeation chromatography (GPC purification, gas chromatography mass spectrometry (GC MS Determination of 33 organochlorine honeysuckle, organophosphorus and pyrethroid pesticide residues. Samples tested pesticide fractions mixed solvent of ethyl acetate cyclohexane (1:1, V / V extraction, GPC purification to remove pigments and other impurities, GC MS using full scan / selected ion monitoring mode (Scan / SIM data collection for qualitative / quantitative analysis. Methods The relative standard deviation (RSD ≤ 16.70% (6.9 ~ 84.6 ng / g, n = 5,5 of different spiked concentrations, the recoveries of pesticides tested in 57.4% (methamidophos ~ 109.9% (Mara parathion between the components of the pesticide detection limit (3σ is 0.10 ~ 10.5 ng / g, quantitative detection limit (10 σ was 0.33 ~ 20.0 ng / g. [Keywords:] honeysuckle, gel permeation chromatography, gas chromatography mass spectrometry, pesticide residues Abstract A method was developed for the simultaneous determination of 33 pesticide residues, including organochlorine, organophosphorus and pyrethroid pesticide, in honeysuckle by gel permeation chromatography (GPC) purification and gas chromatography mass spectrometry (GC MS) detection. The pesticides were extracted using mixed solvent of ethyl acetate and cyclohexane (1:1, V / V). The pesticides extracted from sample were separated and purified with matrices such as pigment by gel permeation chromatography. The pesticides were finally separated and detected by GC MS with full scan and selective ion monitor mode simultaneously. The precisions of developed method with relative standard deviation for 33 pesticide were lower than 16.7% (6.9-84.6 ng / g, n = 5). The recoveries for 33 pesticide standards spiked samples were between