GSL content of ginsenoside Re RP-HPLC method to improve.docVIP

 PAGE \* MERGEFORMAT 8 GSL content of ginsenoside Re RP-HPLC method to improve [Abstract] Objective To improve the high-performance liquid chromatography determination of ginsenosides ginsenosides Re method. The best method of chromatographic conditions: ODS (150 mm * 4.6 mm, 5μ m) for the column, using gradient elution (acetonitrile and water), detection wavelength 203 nm, flow rate 1.0 ml / min. The results of ginsenoside Re in the regression equation Y = 9 505.448 8X-1 159.607 0, r = 0.999 8, the linear range of 1.88 ~ 60.16 μ g / ml, average recovery was 99.35% (RSD = 0.49%, n = 6) . Conclusion The improvement of the determination method is simple, accurate and reproducible, suitable for GSL in the quality control of Ginsenoside Re. [Keywords:] GSL; Effects of ginsenosides Re; RP-HPLC Abstract: ObjectiveTo improve the HPLC method for determination of ginsenosid Re in ginsenosides. MethodsODS column (150 mm * 4.6 mm, 5 μ m) was used. The mobile phase consisted of CH3CN-H2O, the detection wavelength was 203 nm, and the flow rate was 1.0 ml / min.ResultsThe regression equation of ginsenoside Re was Y = 9 505.448 8X-1 159.607 0 with a correlation coefficient of 0.999 8, and linear range was within the range of 1.88 ~ 60.16 μ g / ml, the average recovery of ginsenosid Re was 99.35% (RSD = 0.49%, n = 6). ConclusionThis method is simple, quick, and reproducible and it is suitable for the quality control of Panax ginseng. Keywords:: Panax ginseng; Ginsenosid Re; RP-HPLC Effects of ginsenosides from Panax ginseng extract the dry stems and leaves by the spray-dried active ingredients derived, which contains a variety of glycosides, such as ginsenoside Re. With regard to ginseng saponin ginsenoside Re in the determination method have been reported in the literature [1 ~ 4], I have also used PR-HPLC method of ginsenosides in the content of ginsenoside Re were determined, but some samples of ginsenosides Re there is incomplete separation of the situation. In orde