Health and ketones on rat stomach after carotid artery injury in excessive proliferation of neointima.docVIP

 PAGE \* MERGEFORMAT 16 Health and ketones on rat stomach after carotid artery injury in excessive proliferation of neointima Study: Song Bao, Yu Xuejun, Cui Xin, Chen Jianfei, Lu the spring, Zhao Gang, Yu Yong, Dong Hongmei, Huang Lan [Abstract] Objective: To observe the Health and ketones on the stomach after rat carotid injury neointimal hyperplasia. Methods: Tissue attached method of cultured rat aortic smooth muscle cells, Western blot detection of stomach and ketones on rat aorta Health smooth muscle cells of connexin 43 expression. by balloon injury of rat carotid artery injury model, the control group received normal saline 2 mL, Health and Health gastric stomach ketone ketone group received 3 mg / kg intraperitoneal injection of 1 / d, HE staining neointima formation, neointimal immunofluorescence staining in the expression of connexin 43, Evans blue DAPI double staining to assess neointimal formation. RESULTS: The cultured cells by immunofluorescence anti-SM @ actin staining .50 mol / L students ketone treatment of gastric smooth muscle cells after 24 h the expression of connexin 43 had no effect (0.85 + -0.06 vs 0.83 + -0.03, n = 3, Pgt; 0.05). Balloon Injury in Rats 14 d after carotid artery stenosis visible blood vessels, significant neointimal hyperplasia. immunofluorescence staining showed the formation of neointimal expression of Cx43 in the rich. ketone by the students after the intervention of the stomach significantly reduced neointimal hyperplasia, neointimal cell count was significantly lower than the control group (89 + -28.40 vs 236 + -15.04, n = 5, P lt;0.01). Conclusion: The gap junctions in the neointimal formation after vascular injury play an important role in the process, while the gap junction blocking Health agents can inhibit neointimal ketone gastric hyperplasia. [Keywords:] Neointimal; vessels; gap junction; Health gastric ketone; rat [Abstract] Objective: To observe the effect of carbenoxolone on neointimal hyperpl