Hemolytic streptococcus preparation OK432 combined with tumor antigen induced maturation of bone marrow dendritic cells.docVIP

 PAGE \* MERGEFORMAT 15 Hemolytic streptococcus preparation OK432 combined with tumor antigen induced maturation of bone marrow dendritic cells [Abstract] Objective To investigate the hemolytic streptococci (OK432 combined with tumor antigen induced by bone marrow dendritic cells (DCs mature role to improve the programs mature DCs in vitro for later preparation of DCs for clinical use of anti-tumor vaccine new technical routes. isolated mouse bone marrow mononuclear cells (BMMCs, and containing 10% fetal calf serum (FCS), recombinant mouse granulocyte macrophage colony stimulating factor (rmGM CSF), recombinant mouse interleukin-4 (rmIL 4) of the culture system induced culture, some joined the OK432 and / or tumor antigen impact, observed under light microscopy and transmission electron microscopy morphology, flow cytometry cell surface molecules CD83 and CD86 expression. Results OK432 combined impact of tumor cell lysates typical morphological characteristics of DCs. After the impact of tumor antigen, DCs surface expression of CD83 and CD86 increased, OK432 further stimulation, CD83 and CD86 expression was significantly increased. Conclusion OK432 United mature DCs induced tumor antigen solution is relatively simple, OK432 effectively the impact of tumor antigen after bone marrow DCs further mature. [Keywords:] hemolytic Streptococcus and dendritic cells of bone marrow tumor antigen Dendritic cells (dendritic cells, DCs in vivo is currently the most powerful known antigen presenting cells that have to break the immune tolerance and stimulate tumor-specific tumor immunity and inhibit tumor development. DCs expanded in vitro with the technology increasingly mature, DCs as a cancer treatment cancer vaccine new method shows good prospects for anti-tumor [1]. But for the real DCs used in clinical cancer vaccine, and its in vitro amplification techniques need further optimization. this experiment Streptococcus - OK432 with tumor cell lysates induced marrow-